分析内容
1.差异表达的mRNA列表；
2.统计学分析（含P- value和差异倍数）及聚类分析。
样本类型
细胞，组织，全血，血清，血浆，总RNA等
建议总RNA起始量（单次）：≥ 1μg，浓度：≥50 ng/μL。
近期用户文章
1.tong Q, et al. (2016) LincRNA-Cox2 modulates TNF-a–induced transcription of Il12b gene in intestinal epithelial cells through regulation of Mi-2/NuRD mediated epigenetic histone modifications. FASEB J, 30, 1187-1197 .
2.Guoku Hu,et al.（2016）LincRNA-Cox2 Promotes Late Inflammatory Gene Transcription in Macrophages through Modulating SWI/SNF-Mediated Chromatin Remodeling. The Journal of Immunology. 196 (6) :2799.
3.Feng J, Dai Z, Zhang Y, Meng L, Ye J, Ma X. (2015) Alteration of Gene Expression Profile in Kidney of Spontaneously Hypertensive Rats Treated with Protein Hydrolysate of Blue Mussel (Mytilus edulis) by DNA Microarray Analysis. PLoS ONE 10(10), e0142016.
4.Michael Zorniak, Paul A. Clark, John S. Kuo. (2014) Myelin-forming cell-specific cadherin-19 is a marker for minimally infiltrative glioblastoma stem-like cells. Journal of Neurosurgery. ePub ahead of print doi: 10.3171/2014.9.JNS132373.
5.Baulch JE, Aypar U, Waters KM, Yang AJ, Morgan WF. (2014) Genetic and Epigenetic Changes in Chromosomally Stable and Unstable Progeny of Irradiated Cells. PLoS ONE 9(9).
6.Kamal MM, Sathyan P, Singh SK, Zinn PO, Marisetty AL, Liang S, Gumin J, El-Mesallamy HO, Suki D, Colman H. (2012) REST regulates oncogenic properties of glioblastoma stem cells. Stem Cells 30(3), 405-414.
7.Thomas SN, Waters KM, Morgan WF, Yang AJ, Baulch JE. (2012) Quantitative Proteomic Analysis of Mitochondrial Proteins Reveals Pro-Survival Mechanisms in the Perpetuation of Radiation Induced Genomic Instability. Free Radical Biology and Medicine 53(3), 618-28.
案例展示
LincRNA-Cox2 通过调节SWI/SNF介导的染色质重塑促进巨噬细胞晚期炎症基因的转录
1.研究背景
基因间长链非编码RNA（lincRNAs）是长链非编码转录本（>200 nt），位于蛋白质注释编码基因的间隔区。 最近的研究表明，lincRNA-Cox2可以介导先天免疫细胞中不同类免疫基因的活化和抑制。
2.研究结果
本研究中，研究人员报道lincRNA-Cox2位于1号染色体上PG-内过氧化物合酶2（Ptgs2/ Cox2）基因的近端，是小鼠巨噬细胞中受NF-κB信号控制的早期炎症基因。在功能上，lincRNACox2是在细菌LPS刺激下，受NF-κB调节的晚期炎症反应基因转录所必需的。具体地，lincRNA-Cox2在LPS刺激后，在细胞中被组装进SWI/SNF复合物中。这会导致lincRNA-Cox2/SWI/SNF复合物可以调节NF-κB亚基组装到SWI/SNF复合物中，最终，调节巨噬细胞中SWI/SNF相关的染色质重塑和晚期炎性应答的基因的 反式激活，以应对微生物的挑战。
参考文献
Guoku Hu,et al. LincRNA-Cox2 Promotes Late Inflammatory Gene Transcription in Macrophages through Modulating SWI/SNF-Mediated Chromatin Remodeling. The Journal of Immunology, 2016
